Skip to main content
. 2011 Dec 21;40(8):3456–3469. doi: 10.1093/nar/gkr1242

Figure 3.

Figure 3.

Ku proteins do not affect significantly Cdx2 transcriptional activity. (A) Stimulation of endogenous Cdx2 target genes. HCT116 were transfected with 1 µg of each plasmid in a combined manner as indicated and RNA were extracted and analyzed by RT-qPCR using CDH17, SI or TBP TaqMan probes. Results are represented as fold induction relative to pFlag (normalized with TBP expression). Data correspond to the mean of several experiments and error bars represent SEM (n = 2). (B) Stimulation of Cdx2 reporter plasmids. HCT116 were transfected with CDH17-luc or SI-luc reporter plasmids and with indicated expression plasmids. Protein extracts were analyzed for dual luciferase activity. Relative promoter activity (normalized with Renilla values) of reporter plasmid cotransfected with empty plasmids was set at 1. Data correspond to the mean of several experiments and error bars represent SEM (n = 3). Right panel: FOXO4 transcription factor was used as positive control for Ku70 inhibition on the 6DBE-luc reporter plasmid as described in Brenkman et al. (27).