Figure 2.

αSSE and CSE activate transcription cooperatively. (A) The expression profile of all the Pcdhα genes in HEC-1B, 293T and SH-SY5Y cell lines by RT–PCR, (Supplementary Figure S2). Gray and black boxes indicate for absence or presence of Pcdhα mRNA, respectively. (B) A scheme depicting the sequences of α6SSE–CSE WT and mutants (b–d). (C) WT (α6SSE–CSE and α3SSE–CSE) and the mutated promoters (fused to firefly luciferase reporter gene) were transfected into HEC-1B, 293T and SH-SY5Ycell lines together with RSV-Renilla that serves as internal control. The parental pGL3-basic (Basic) was also transfected as a control. Twenty-four hours post-transfection firefly and renilla luciferase activities were measured. The normalized results are the mean of at least four independent experiments (±SD).