Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Dec 30;40(8):3574–3584. doi: 10.1093/nar/gkr1279

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — Lin28 binds Oct4-95 in vitro. Autoradiographs of EMSA using labeled Oct4-95 (A) or pre-let-7a RNA (B) with increasing amounts of rLin28. The positions of unbound RNA– and RNA–protein complexes are marked to the right. Band intensities were quantified from three independent experiments and used to calculate the K_d values shown in the plots below. In the case of Oct4-95, Complex 1 and 2 were combined as one complex in K_d calculation. (C) Autoradiograph of EMSA performed with labeled pre-let-7a RNA and 3.2 µM of rLin28. The amounts of unlabeled competitor Oct4-95 or pre-let-7a RNA added in relative molar excess are indicated on the top. Band intensities were calculated and presented as percentage of fraction bound. Numbers are averages of two independent experiments.