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Comparison of steady state kinetic parameters for Gln4 and Gln4 variants
| kcat (s−1) | KMtRNA (µM) | kcat/KMtRNA (M−1·s−1) | KMGln (mM) | kcat/KMGln (M−1·s−1) | |
|---|---|---|---|---|---|
| FL-Gln4 | 1.4 ± 0.2 | 0.19 ± 0.04 | 7.6 × 106 | 1.7 ± 0.2 | 8.5 × 102 |
| Gln4 (187–809) | 1.7 ± 0.3 | 5.85 ± 0.52 | 2.9 × 105 | 9.3 ± 0.3 | 1.8 × 102 |
| PVG-GlnRS | 2.8 ± 0.6 | 1.55 ± 0.51 | 1.8 × 106 | NA | NA |
| FL-Gln4 + native tRNA | 1.7 ± 0.1 | 0.14 ± 0.07 | 1.2 × 107 | NA | NA |
