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. 2011 Dec 30;40(8):3585–3595. doi: 10.1093/nar/gkr1280

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Effects of Btr and Btr:BB on open complex formation. Open complex (RP_O) formation was monitored by KMnO₄ footprinting on negatively supercoiled DNA. Lane 1 is an A+G chemical sequencing ladder (for indexing) and lane 2 is a reaction that contains only plasmid DNA. RNAP (100 nM), Btr (50 nM), BB (200 nM) and Fur (50 nM) were added as indicated. Chemical sensitivity within the transcription bubble (e.g. −7,−8) in lanes 3 and 4 was normalized by comparison to the background reactivity in the +18 to +21 region in lanes containing Fur protein (5–7).