Figure 6.

(A) Schematic representation indicating size and composition of SdsR variants or chimeras tested for their impact on OmpD expression. TMA designates a 5′ shortened variant of the unrelated MicA sRNA (Truncated MicA). For sequences of the respective constructs, see Supplementary Figure S6. (B) The SdsR 5′-end is required for ompD regulation. Salmonella ΔsdsR transformed with a control vector or plasmids constitutively expressing versions of SdsR (as depicted in A; pP_L-SdsR; pP_L-SdsR proc.; pP_L-SdsR +7; pP_L-SdsR +19; pP_L-SdsR-TMA; pP_L-TMA; see Supplementary Table S2 for details on plasmids) were grown to an OD₆₀₀ of 2.0. Total protein samples were analysed by SDS-PAGE (upper panel) and specific deregulation of OmpD was confirmed by western blotting (lower panel). (C) Expression of ompD mRNA in the presence of control RNAs or the various SdsR constructs used in (B) was determined by northern blotting; probing of 5S rRNA served as loading control.