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. 2011 Dec 16;40(8):3623–3640. doi: 10.1093/nar/gkr1156

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — (A) Predicted duplex forming between SdsR sRNA and ompD mRNA. Point mutations to generate the compensatory ompD* and SdsR* alleles are indicated.(B) Compensatory base pair exchanges validate the SdsR–ompD interaction. Salmonella ΔsdsR ompD* mutant (JVS-9155) or isogenic ΔsdsR ompD (JVS-9154) cells carrying plasmids for the constitutive overexpression of either SdsR (pKF68-3) or SdsR* (pKF101-26), respectively, were grown to an OD₆₀₀ of 2.0. Expression levels of ompD/ompD* mRNAs and SdsR/SdsR* sRNAs were determined by northern blot analysis of total RNA; probing of 5S rRNA served as loading control.(C) OmpD levels with respect to SdsR or SdsR* expression were analysed by SDS–PAGE (upper panel) and western blot (lower panel) using total protein samples prepared in parallel to the RNA samples in (B). OmpD protein is indicated by an arrow.