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Dissociation of immune complexes in rheumatoid arthritis fluid. An aliquot of rheumatoid arthritis fluid was acidified to dissociate immune complexes, then fractionated on a C-4 column before Western immunoblotting (lane A). Another aliquot of the same sample (lane B) was immunoaffinity-purified as in figure 2 but was not subjected to immune complex dissociation.
