Table 3.
Genes differentially expressed in naïve (day 0) DA versus E3 rats
| Accession no. | Gene ID | Significance | Affymetrix | Custom-made | FACS | ELISA |
| Upregulated | ||||||
| X56596 | MHC class II antigen RT1.B-1 β-chain (MhcII) | T | I (56.8) | NC | I (1.2); P < 0.08 | ND |
| X00923 | IgE heavy chain (Ig ε) | S | I (3.6) | I (1.9); P < 0.1 | ND | I (1.4); P < 0.05 |
| D49955 | Bone marrow stromal cell antigen 1 (Bst1) | T | I (3.7) | I (2.0); P < 0.1 | ND | ND |
| Downregulated | ||||||
| S81289 | IgM κ chain variable region (CDR1 to CDR3 region; Ig κ) | S | D (-3.6) | D (-1.6);P < 0.0005 | NC | ND |
To be regarded as differentially expressed a gene must be differentially expressed in two biological samples analyzed by a minimum of two independent methods. Differential expression values in bold text are statistically significant by that particular method. Genes significantly differentially expressed by a minimum of two independent methods are denoted 'S'. Another subset of genes showed a strong tendency toward differential expression; these are denoted 'T'. ELISA, enzyme linked immunosorbent assay; FACS, fluorescence activated cell sorting; NC, no change (for these genes, no statistically significant differential expression could be shown); ND, not determined.