Table 4.
Genes differentially expressed in DA versus E3 rats 8 days after pristane injection
| Affymetrix | |||||||
| Accession no. | Gene ID | Significance | Pooled | Individual | Taqman | Custom-made | FACS |
| Upregulated | |||||||
| X56596 | MHC class I antigen RT1.B-1 β-chain (MhcII) | S | I (47.4) |
I (43.4); P < 0.0001 |
ND | I (2.8); P < 0.1 |
I (1.9); P < 0.03 |
| U24441 | Metalloproteinase 9 (Mmp9) | T | I (5) |
I (1.5); P < 0.001 |
I (1.9); P < 0.08 |
NC‡ | ND |
| S81478 | CL100 protein tyrosine phosphatase (Ptpn16) | T | I (2.4) | NC* | ND |
I (1.6); P < 0.02 |
ND |
| Downregulated | |||||||
| L07399 | (Hybridoma 56R-7) immunoglobulin rearranged γ-chain variable (Ig γ) |
S | D (-6.4) | NC* | ND |
D (-2.0); P < 0.02 |
ND |
| L07401 | (Hybridoma 57R-1) immunoglobulin rearranged γ-chain variable (Ig γ) |
S | D (-62.4) | NC* |
D (-2.1); P < 0.03 |
NC‡ | ND |
| M14434 | Immunoglobulin active κ-chain VJC region from immunocytoma IR2 (Ig κ) |
S | D (-34.7) | NC* | ND |
D (-1.9); P < 0.003 |
ND |
| X00923 | IgE heavy chain (Ig ε) | S | D (-28) | NC* | ND |
D (-2.0); P < 0.05 |
ND |
| S81289 | IgM κ-chain variable (Ig κ) | S | D (-9.3) | NC* | ND |
D (-2.5); P < 0.0003 |
ND |
| M15402 | Immunoglobulin active κ-chain VJ region from immunocytoma IR162 (Ig κ) |
S | D (-3.9) | NC* | ND |
D (-3.0); P < 0.006 |
ND |
| nm_013121 | CD28 (Cd28) | S | NC | NC* | ND |
D (-2.2); P < 0.01 |
D (-9.8); P < 0.03 |
| S69206 | Mast cell protease 1 (Mcpt1) | S | D (-4.7) | NC* | ND |
D (-2.1); P < 0.002 |
ND |
| AB010635 | Carboxylesterase precursor (Ces2) | S | D (-4.1) | NC* | ND |
D (-2.5) ; P < 0.003 |
ND |
| D25290 | K-cadherin (Cdh6) | S | D (-4.1) | NC* | ND |
D (-1.7) ; P < 0.01 |
ND |
| X70871 | Cyclin G1 (Ccng1) | S | D (-9.6) |
D (-4.8); P < 0.001 |
D† | NC‡ | ND |
| AJ011608 | DNA polymerase α-subunit IV (Primase) | S | D (-3.3) |
D (-2.6); P < 0.001 |
D (-3.5); P < 0.03 |
NC‡ | ND |
| L12025 | Tumour-associated glycoprotein E4 (Tage) | T | D (-3.1) | NC* | ND | D (-1.8); P < 0.1 |
ND |
To be regarded as differentially expressed a gene must be differentially expressed in two biological samples analyzed by a minimum of two independent methods. Differential expression values in bold text are statistically significant by that particular method. Genes significantly differentially expressed by a minimum of two independent methods are denoted 'S'. Another subset of genes showed a strong tendency toward differential expression; these are denoted 'T'. FACS, fluorescence activated cell sorting; NC, no change; ND, not determined. *These genes were not included among the 30 genes with highest probability of differential expression (t-test P < 0.002). †This gene was only detectable in the E3 rat. ‡For these genes, no statistically significant differential expression could be shown.