Fig. 2.
Phosphoinositide 3-kinase (PI3K) activity in mouse pulmonary microvascular ECs (PMVECs) is increased with ischemia. Flow-adapted WT, ATP-sensitive K+ (KATP) channel (Kir6.2)-null, or NADPH oxidase [NOX2; glycoprotein (gp)91phox]-null cells were subjected to stop of flow. Cells were immediately removed from the cartridges, lysed, incubated with anti-p85 antibody to immunoprecipitate PI3K, and assayed for PI3K activity with phosphatidylinositol (4,5)-biphosphate (PIP2) as the substrate. WT cells cultured under static conditions were the control. *P < 0.01 vs. control cells.