Fig. 1.
Characteristics of the mitofusin-1 (Mfn-1) knockout (KO) heart. A: Western blot analysis performed to detect endogenous levels of Mfn-1 and dynamin-related protein-1 (Drp-1) in mouse heart extracts. GAPDH is used as a loading control. B: Western blot analysis performed to detect cre-specific reduction of Mfn-1 in the heart. Porin/VDAC1 is used as a mitochondria loading control (WT, wild-type; HET, heterozygous for Mfn-1flox; KO, homozygous for Mfn-1flox and positive for cre). C: gross appearance of hearts from 5-mo-old Mfn-1 WT and KO mice; scale bar = 2 mm. D: size of hearts is reported as the ratio of heart weight (HW) to tibia length (TL; n = 6 WT; 4 mice were homozygous Mfn-1flox without cre and 2 were homozygous Mfn-1+/+ with cre and n = 5 mice homozygous Mfn-1flox with cre).