Table 1.
Transcriptional regulation of relevant genes
| Transcript | Fold Induction/Reduction (Mfn-1 KO vs. WT) | P | Functional Class |
|---|---|---|---|
| Mfn-1 | 0.074 | 0.001 | Mitochondrial fusion/fission |
| Mfn-2 | 0.838 | 0.017 | |
| Opa-1 | 0.796 | 0.040 | |
| Drp-1 | 0.805 | 0.041 | |
| Mtp-18 | 1.047 | 0.587 | |
| Slp-2 | 1.028 | 0.721 | |
| Tfam | 0.516 | 0.098 | Mitochondrial biogenesis |
| Pgc-1α | 0.409 | 0.078 | |
| Err-α | 1.250 | 0.307 | |
| Endo-G | 0.768 | 0.018 | MTDNA maintenance |
| Pol-γ | 1.002 | 0.986 | |
| Ndufb-5 | 0.744 | 0.040 | ETC/ATP synthase |
| Nd-5 | 0.647 | 0.033 | |
| Cox4-1 | 0.609 | 0.026 | |
| Cox4-2 | 0.718 | 0.090 | |
| Cox-5b | 0.937 | 0.261 | |
| Atp-5o | 0.640 | 0.067 | |
| Anp | 5.699 | 0.001 | Stress indicators |
| Bnp | 5.427 | 0.001 | |
| β-Mhc | 0.793 | 0.058 | |
| α-Sk.actin | 1.100 | 0.738 | |
| Sod-2 | 0.676 | 0.007 | ROS detoxification |
| Bcl-2 | 1.345 | 0.001 | Apoptosis/autophagy |
| Beclin-1 | 0.865 | 0.353 | |
| LC-3b | 0.843 | 0.126 |
Values represent fold reduction/induction in the mitofusin-1 (Mfn-1) knockout (KO) group (n = 5) relative to wild type (WT; n = 4). Comparisons for statistical significance were performed with two-tailed Student's t-test.
Mfn-2, mitofusin 2; Opa-1, optic atrophy 1 long variant; Drp-1, dynamin-related protein 1; Mtp-18, mitochondrial protein 18; Slp-2, stomatin like protein 2; Tfam, transcriptional factor A mitochondrial; Pgc-1α, PPAR-γ coactivator 1α; Err-α, estrogen receptor related factor-α; Endo-G, endonuclease-γ; MTDNA, mitochondrial DNA; Pol-γ, polymerase-γ; Ndufb-5, NADH-dehydrogenase (ubiquinone) subcomplex 1β, polypeptide 5; Nd-5, NADH-dehydrogenase subunit 5, mitochondrially encoded; Cox4-1, cytochrome c oxidase, subunit 4, isoform 1; Cox4-2, cytochrome c oxidase, subunit 4, isoform 2; Cox-5b, cytochrome c oxidase subunit 5b; Atp5o, F1FO-ATPase complex, oligomycin sensitivity-conferring subunit, polypeptide 5; Anp, atrial natriuretic peptide; Bnp, brain natriuretic peptide; β-MHC, β-myosin heavy chain; α-Sk actin; α-skeletal actin; Sod2, superoxide dismutase, mitochondrial; LC-3b; microtubule associated protein 1 light chain 3b; ETC, electron transport chain; ROS, reactive oxygen species.