Fig. 2.

TES increases NO production in porcine and human CASM. A: average nitrite accumulation in porcine CASM before (con) and 30 min after 10 μM TES and in the presence of 20 μM l-NMMA or l-NMMA and 2 mM l-arginine (l-arg). Bars represent mean values ± SE (n = 4). P < 0.05 compared with control (*) and compared with TES alone (#). B: 4-amino-5-methylamino- 2′,7′-difluorescein (DAF-2) fluorescence recorded from human CASM myocytes before (con) and 20 min after 100 nM TES. The time course plot is a moving average of fluorescence intensity after addition of TES. C: DAF-2 fluorescence recorded from human CASM myocytes in the presence of 10 μM N^ω-propyl-l-arginine (l-NPA) and then 20 min after subsequent addition of 100 nM TES. The time course plot is a moving average of fluorescence intensity in the presence of l-NPA and TES normalized to control levels (i.e., 1.0). Inset: immunoblot detection of nitric oxide synthase isoforms expressed in human CASM (representative of n = 4 preparations). eNOS, endothelial nitric oxide synthase; nNOS, neuronal nitric oxide synthase.