Fig. 3.
A: the stimulatory effect of TES on BKCa channel activity is not easily reversed. Representative recordings (+40 mV) before (con) and 10 min after exposure to 100 nM TES and then 20 min after washout (w/o) of TES. Channel activity was only minimally reversed even 20–60 min after TES removal (n = 3 cell-attached patches). B: TES-stimulated BKCa channel activity is inhibited by wortmannin. Summary analysis of channel open probability (NPo) before (con) and 10 min after exposure to 100 nM TES. In the presence of 50 nM wortmannin (Wt), TES had no significant effect on channel activity (n = 6; *P < 0.05 compared with control). Each bar represents the mean channel NPo ± SE.