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. 2010 Aug;24(6):996–1007. doi: 10.1016/j.bbi.2010.04.004

Fig. 4.

Fig. 4

Microglia are primed with respect to responses to systemic poly I:C. Microglial priming and microglial/endothelial activation was assessed 3 h after poly I:C or saline in NBH and ME7 animals. (a–c) IBA-1 labelling of microglial cells in NBH + poly I:C, ME7 + saline and ME7 + poly I:C animal groups, showing more condensed morphology and increased numbers in ME7 with respect to NBH. (d–f) COX-2 labelling shows few microglia in NBH animals but activated endothelium after poly I:C. Conversely, ME7 + saline shows increased microglia but absent endothelial staining, while ME7 + poly I:C animals show both populations are COX-2-positive. (g–i) IL-1β labelling is absent in both NBH + poly I:C and ME7 + saline animals but ME7 + poly I:C (i) shows positive cells of microglial morphology (inset) in periventricular and perivascular areas and around the dentate gyrus. (j–l) IRF3 labelling in scattered cells in NBH + poly I:C and in ME7 + saline and in greater numbers in periventricular areas and around the dentate gyrus in ME7 + poly I:C animals. Scale bar = 100 μm in (a–c) and (g–i) and 50 μm in (d–f) and (j–l).