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. 2003 May 1;23(9):3844–3854. doi: 10.1523/JNEUROSCI.23-09-03844.2003

Fig. 1.

Fig. 1.

Patch-clamp recording from gastric-related rat DMV neurons prelabeled with PRV-152. A, Whole-mount view of a brainstem slice (400 μm) after fixation reveals EGFP-labeled DMV neurons 70 hr after inoculation of the stomach wall. The neuron indicated (arrow) was targeted for recording and filled with biocytin.B, The same slice and plane of section viewed with optics demonstrating the biocytin label (i.e., avidin–rhodamine fluorescence). The filled neuron is indicated by the arrow. Inset, Examples of spontaneous EPSCs from this neuron and IPSCs from another PRV152-labeled DMV neuron. C, The same neuron in whole mount after ABC–DAB reaction. D, The same neuron was reconstructed digitally. Arrows point to the axon.