Fig. 5.

The effect of hypocretin on spontaneous EPSCs in the DMV. A, Spontaneous EPSCs were observed in this neuron at a holding potential of −75 mV. B, The same neuron in the presence of hypocretin 2 (1 μm).C, Twenty minute wash to normal ACSF. This cell was prelabeled by inoculation of the stomach wall with PRV-152.D, Cumulative fraction plot indicates no significant change in sIPSC frequency by hypocretin 2. E, Plots for frequency and amplitude changes after hypocretin 1 and 2 application (1 μm) for several neurons. No significant changes in EPSC amplitude or frequency were observed for either peptide.