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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Cell Signal. 2011 Dec 29;24(4):931–942. doi: 10.1016/j.cellsig.2011.12.016

Fig. 5. Spermine interacts directly with Src and associates preferentially with the SH2 domain.

Fig. 5

A, various domains of mouse neuronal c-Src B, ELISA-based pair-wise binding of GST-Src with BSA-conjugated spermine (n=3). BSA-conjugated spermine was incubated with GST or GST-Src immobilized in the ELISA wells, followed by the incubation with anti-spermine serum. The absorbance was measured at 450 nm. C, PCR-cloned His-S-tagged or GST-tagged Src domain proteins resolved by SDS-PAGE. D, pair-wise binding between His-S-SH2 and HisS-SH3 domains of Src with BSA-spermine. The mixture was pulled down using S-beads, and blotted with anti-spermine serum. Representative blots from three independent observations are shown. E, pair-wise binding between BSA-spermine and different doses of GST-His-S-SH2 domain of Src. The mixture was pulled down using glutathione beads, and blotted with anti-spermine serum. A representative blot from three independent observations is shown.