Skip to main content
. Author manuscript; available in PMC: 2013 Apr 18.
Published in final edited form as: J Am Chem Soc. 2012 Apr 9;134(15):6865–6877. doi: 10.1021/ja3016389

Figure 6.

Figure 6

The editing function of PksA TE. A) 14C-acyl-ACP hydrolysis assay toward hexanoyl, acetyl, and malonyl species. 10 μM acyl-ACP was treated with no TE, TE-S1937A (3 μM), or TE (3, 1, 0.33, 0.11, 0.04, or 0.01 μM) for 6 min. Proteins were separated by SDS-PAGE and phosphorimaged. B) Kinetic constants for TE catalyzed acyl-SNAC hydrolysis from a continuous UV-vis spectrophotometric assay using Ellman’s reagent. C) PksA product titers are dependent on TE concentration. Constant SAT-KS-MAT and ACP (10 μM each) were combined with variable TE (0, 0.1, 0.5, 2, 5, 10, 20, or 40 μM).