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. Author manuscript; available in PMC: 2013 Apr 18.
Published in final edited form as: J Am Chem Soc. 2012 Apr 9;134(15):6865–6877. doi: 10.1021/ja3016389

Figure 8.

Figure 8

Transacylase activities of PksA domains. A) RPLC-based transacylase assay. SAT monodomain hexanoyl transfer rate was previously determined.24 B) 14C-acyl-CoA transacylase assay. SAT-KS-MAT (144 kDa) and PT-ACP (59 kDa) were treated with hexanoyl (top panel) or malonyl (lower panel). The symbol 0 denotes a loss-of-function mutant which has the active site nucleophile removed by mutation to an alanine.