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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: FEMS Microbiol Lett. 2012 Jan 30;328(2):174–181. doi: 10.1111/j.1574-6968.2012.02496.x

Figure 2. Localization of YgfX in the inner membrane.

Figure 2

E. coli BL21 (DE3) cells were transformed with pBAD24-ygfX (lane 2, 4, 6, and 8) or with pBAD24 (lane 1, 3, 5, and 7), and treated with 0.2% arabinose for 1 hr. The soluble (S) fractions (lane 1 and 2) and the membrane (M) fractions (lane 3 and 4) were separated by ultracentrifugation at 100,000 x g for 1 hr. The membrane fraction was resuspended in 1% N-laurylsarcosine (Hobb et al., 2009). The detergent-soluble inner membrane (IM; lane 5 and 6) and the insoluble outer membrane (OM; lane 7 and 8) were separated by ultracentrifugation as described above. Proteins were separated by 17.5% SDS-PAGE 447 and visualized by Coommassie blue staining.