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. Author manuscript; available in PMC: 2013 Apr 10.
Published in final edited form as: Regul Pept. 2012 Jan 23;175(1-3):49–60. doi: 10.1016/j.regpep.2012.01.006

Figure 5. PTH1R levels in acinar and stellate cells treated with cerulein or ethanol.

Figure 5

(A) Primary (10) acinar and stellate cells, AR42J (acinar) and iPSCc3 (stellate) cells were treated with cerulein (10−7 M) or ethanol (50 mM) for 4 h. Saos-2 cells were used as positive control. PTH1R mRNA levels were measured by reverse transcription/real-time PCR. Values are expressed relative to the Saos-2 value, set arbitrarily at 100. Each bar is the mean ± SEM of three independent experiments. (B and C) IHC analysis for PTH1R in cells treated with cerulein (CR) or ethanol (EtOH) as described in (A). − = no treatment. IgG = antibody control. The panels other than IgG were immunostained with anti-PTH1R antibody.