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. Author manuscript; available in PMC: 2012 Dec 9.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Nov 11;416(1-2):153–158. doi: 10.1016/j.bbrc.2011.11.015

Figure 4.

Figure 4

Effect of ISG15 and UBP43 knockdown on R. conorii replication in HMECs. HMECs were transfected with ISG15 and UBP43-specific siRNA or non-target control siRNA prior to infection with R. conorii. To ensure the efficacy of knockdown, the levels of ISG15 (A) and UBP43 (B) were first investigated by Western blot analysis. Next, the levels of intracellular replication of R. conorii in HMECs transfected with siRNAs against ISG15 and UBP43 were determined by rickettsial outer membrane protein A (rOMPA)-based quantitative PCR. Rickettsial replication was significantly increased in ISG15-knockdown cells (C), but was significantly lower than the corresponding controls in UBP43-siRNA transfected cells (D).