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SRSF1 knockdown impairs anchorage-independent growth of MYC-transformed cells. (A) Immunoblotting of MYC and SRSF1 in the Rat1a-pBabe-Luc control cell line, Rat1a-MYC, and Rat1a-MYC cells transduced with one of two shRNAs against SRSF1. (B) Growth curves of the four cell lines from (A). Error bars, s.d.; n=3. (C) Anchorage-independent growth of cell lines from (A) in soft-agar colony-formation assays. Error bars, s.d.; n=3; t-test *P<0.05.
