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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Nat Protoc. 2012 Mar 1;7(3):562–578. doi: 10.1038/nprot.2012.016

Figure 10.

Figure 10

Differential analysis results for Rala. (a) This gene has four isoforms in the merged assembly. (b) Cuffdiff identifies TCONS_00024713 and TCONS_00024715 as being significantly differentially expressed. The relatively modest overall change in gene-level expression, combined with high isoform-level measurement variability, leaves Cuffdiff unable to reject the null hypothesis that the observed gene level is attributable to measurement or cross-replicate variability.