Fig. 2.

Effects of 1,25(OH)₂D₃ on the STC1 mRNA expressions in OK-P cells. When OK-P cells reached confluence, the cells were treated with the control (final concentration of ethanol, 0.1%) or (A) 100 nM 1,25D₃ for 6 h, or multiple concentrations of (B) 1,25D₃ for 6 h, or (C) 100 nM 1,25D₃ for time course study. (D) The cells were also incubated with the control, 100 nM 1,25D₃, OCT or ED-71 for 6 h. RNA was extracted from cells at the indicated time points, and RT-PCR or real-time RT-PCR was performed to evaluate STC1 and STC2 expression. Results were normalized to the mRNA expression level of β-actin. The data are represented as the means ± SD (n = 3). *p<0.05, **p<0.001 compared with control.