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. Author manuscript; available in PMC: 2013 Apr 20.
Published in final edited form as: Immunity. 2012 Apr 5;36(4):561–571. doi: 10.1016/j.immuni.2012.02.014

Figure 1. The HIN:DNA Interactions are Non-sequence Specific and Sensitive to Ionic Strength.

Figure 1

(A) Fluorescence polarization (FP) assays of the FAM-labeled dsDNAs of various sequences and GC contents upon binding to the AIM2 HIN domain. The apparent Kd values (Kda) are shown for each dsDNA.

(B) FP assays of the FAM-labeled dsDNA ODN787/788 upon binding to the AIM2 HIN domain in the presence of various concentrations of sodium chloride.

(C) FP assays of the FAM-labeled dsDNA ODN787/788 upon binding to the IFI16 HINb domain in the presence of various concentrations of sodium chloride.