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Preparation of SP1572-PdT-Vi (A) and SP2070-PdT-Vi (B) conjugates. SP1572-PdT and SP2070-PdT were purified from E. coli and then conjugated to Vi as described in Materials and Methods. Products were run through a Superose 6 column and fractions from void volume (which contain conjugated material) were collected to separate conjugate from free proteins.
