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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Exp Neurol. 2011 Dec 29;235(1):162–173. doi: 10.1016/j.expneurol.2011.12.037

Table 1. Experimental groups for in vivo studies on regeneration of CTB labeled axons.

All animals received C3 dorsal column wire knife lesions and BMSC grafts mixed with NT-3 protein. Lentivirus for expression of NT-3 or GFP were injected at the same time rostral to the lesion site. Rolipram and mesopram infusions (2.6 mg/kg/day) were started 1 week prior to C3 lesions, pumps were exchanged at the time lesions were made, and infusions continued for one additional week. Conditioning lesions were done 1 week prior to C3 dorsal column lesions (preconditioning) or 1 or 7 days after C3 lesions (post-conditioning). Injections of cAMP or PBS into L4, 5 DRGs was done 1 day after C3 lesions.

Groups Conditioning/Infusion/Injection Rostral vector Number of animals for CTB labeling
1 No treatment GFP n=6
2 No treatment NT-3 n=6
3 Pre-Conditioning lesion GFP n=6
4 Pre-Conditioning lesion NT-3 n=6
5 Rolipram infusion GFP n=6
6 Rolipram infusion NT-3 n=6
7 Mesopram infusion No vector n=6
8 Mesopram infusion NT-3 n=6
9 1 day Post-Conditioning lesion NT-3 n=7
10 7 day Post-Conditioning lesion NT-3 n=7
11 cAMP injection NT-3 n=6
12 PBS injections NT-3 n=6