Table 6. qRT-PCR data confirming significant changes of selected genes in microarrays in DRGs of animals after mesopram infusions and conditioning lesions at day 1.

Values are log2 changes (% of control in parentheses) of gene expression compared to controls (naïve animals and animals with DMSO infusions, respectively).

	Conditioning Lesion			Mesopram Infusion
Gene	Identifier	Microarray	qRT-PCR	Microarray	qRT-PCR
Cebp/d	1368813_at 1387343_at	1.82 (353%)^** 1.52 (286%)^**	2.06 (417%)^*	0.63 (155%)^NS 0.61 (153%)^*	0.59 (151%)^#
Egr1	1368321_at	1.00 (200%)^****	1.31 (247%)^**	0.93 (191%)^**	0.91 (187%)^**
Egr3	1392791_at	0.95 (193%)^****	1.79 (346%)^**	1.07 (210%)^*	0.43 (134%)^NS
ATF3	1369268_at	5.07 (3359%)^****	6.17 (7201%)^***	0.46 (138%)^NS	1.55 (295%)^NS
Myc	1368308_at	1.26 (239%)^****	1.96 (388%)^*	−0.01 (99%)^NS	0.15 (111%)^NS
Jun	1389528_s_at 1374404_at 1369788_s_at	1.47 (277%)^** 1.59 (301%)^ 1.40 (264%)^**	1.05 (207%)^*	−0.4 (76%)^NS −0.54 (69%)^NS −0.14 (91%)^NS	−0.45 (73%)^NS
Fos	1375043_at	1.50 (283%)^***	2.99 (792%)^****	1.03 (204%)^***	1.01 (201%)^*
Smad1	1369174_at 1389373_at 1396061_at	0.97 (196%)^* 0.93 (191%)^** 0.02 (101%)^NS	1.56 (294%)^**	−0.1 (93%)^NS −0.12 (92%)^NS 0.13 (109%)^NS	−0.26 (83%)^NS
Sox11	1371450_at 1387275_at	2.21 (463%)^** 2.55(586%)^**	3.54 (1164%)^***	0.23 (117%)^NS 0.33 (126%)^NS	0.01 (101%)^NS
GADD45A	1368947_at	2.60(606%)^****	4.0 (1589%)^**	0.08 (106%)^NS	nd
GADD45g	1388792_at	1.93(381%)^****	3.2 (926%)^***	0 (100%)^NS	nd
ZFP367	1379967_at	1.22 (233%)^****	3.06 (836%)^***	−0.12 (92%)^NS	nd
NFIL3	1368488_at	1.39 (262%)^****	1.59 (300%)^**	0.25 (119%)^NS	nd

Some genes are represented by more than a single probe set;

^NS

not significantly different from control;

p<0.05;

^**

p<0.01,

^***

p<0.001;

^****

p<0.0001,

p=0.07,

nd: not determined