Table 6. qRT-PCR data confirming significant changes of selected genes in microarrays in DRGs of animals after mesopram infusions and conditioning lesions at day 1.
Conditioning Lesion | Mesopram Infusion | ||||
---|---|---|---|---|---|
Gene | Identifier | Microarray | qRT-PCR | Microarray | qRT-PCR |
Cebp/d | 1368813_at 1387343_at |
1.82 (353%)**** 1.52 (286%)**** |
2.06 (417%)* | 0.63 (155%)NS 0.61 (153%)* |
0.59 (151%)# |
Egr1 | 1368321_at | 1.00 (200%)**** | 1.31 (247%)** | 0.93 (191%)** | 0.91 (187%)** |
Egr3 | 1392791_at | 0.95 (193%)**** | 1.79 (346%)** | 1.07 (210%)* | 0.43 (134%)NS |
ATF3 | 1369268_at | 5.07 (3359%)**** | 6.17 (7201%)*** | 0.46 (138%)NS | 1.55 (295%)NS |
Myc | 1368308_at | 1.26 (239%)**** | 1.96 (388%)* | −0.01 (99%)NS | 0.15 (111%)NS |
Jun | 1389528_s_at 1374404_at 1369788_s_at |
1.47 (277%)**** 1.59 (301%)**** 1.40 (264%)**** |
1.05 (207%)* | −0.4 (76%)NS −0.54 (69%)NS −0.14 (91%)NS |
−0.45 (73%)NS |
Fos | 1375043_at | 1.50 (283%)*** | 2.99 (792%)**** | 1.03 (204%)*** | 1.01 (201%)* |
Smad1 | 1369174_at 1389373_at 1396061_at |
0.97 (196%)*** 0.93 (191%)**** 0.02 (101%)NS |
1.56 (294%)** | −0.1 (93%)NS −0.12 (92%)NS 0.13 (109%)NS |
−0.26 (83%)NS |
Sox11 | 1371450_at 1387275_at |
2.21 (463%)**** 2.55(586%)**** |
3.54 (1164%)*** | 0.23 (117%)NS 0.33 (126%)NS |
0.01 (101%)NS |
GADD45A | 1368947_at | 2.60(606%)**** | 4.0 (1589%)** | 0.08 (106%)NS | nd |
GADD45g | 1388792_at | 1.93(381%)**** | 3.2 (926%)*** | 0 (100%)NS | nd |
ZFP367 | 1379967_at | 1.22 (233%)**** | 3.06 (836%)*** | −0.12 (92%)NS | nd |
NFIL3 | 1368488_at | 1.39 (262%)**** | 1.59 (300%)** | 0.25 (119%)NS | nd |
Some genes are represented by more than a single probe set;
not significantly different from control;
p<0.05;
p<0.01,
p<0.001;
p<0.0001,
p=0.07,
nd: not determined