Fig. 1.

Genotypes of the g.32373708 G>A-IRP1 polymorphism (rs867469) determined by the allele-specific oligonucleotide-PCR (ASO-PCR) method and analyzed by a 8% polyacrylamide gel electrophoresis stained with ethidium bromide and viewed under UV light. Lane M displays GeneRuler ^™ 100 bp molecular weight marker, lanes designated G or A show the results of amplification with primer specific to either the G or A allele, respectively and lane X shows a negative control comprising reaction mixture without target DNA. Genotypes are indicated in the lower part of the picture