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. 2012 Apr 23;7(4):e35717. doi: 10.1371/journal.pone.0035717

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Transient cotransfections were performed in subconfluent HCT116 cells using wild-type and deletion mutants of GATA4 and wild-type PIAS1 (panel A). In panel B, wild-type and deletion mutants of PIAS1 and wild-type GATA4 were used for cotransfections. Lysates were assayed for luciferase activity 48 hours post-transfection. Results from 3 experiments done in triplicates are shown as mean±SEM.