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. 1990 Feb 11;18(3):513–520. doi: 10.1093/nar/18.3.513

Adenovirus and minute virus of mice DNAs are localized at the nuclear periphery.

P T Moen Jr 1, E Fox 1, J W Bodnar 1
PMCID: PMC333456  PMID: 2408013

Abstract

The localization of adenovirus 2 (Ad2) and Minute Virus of Mice (MVM) DNAs was studied in situ in infected HeLa cell nuclei using fluorescent DNA probes and confocal microscopy. Ad2 DNA was found in multiple foci which were localized along the periphery of the infected cell nuclei. MVM DNA was found in HeLa cell nucleoli which are associated with the nuclear envelope, and when co-infected with Ad2 MVM DNA was compartmentalized to multiple foci which again were localized at the nuclear periphery. The data are discussed in terms of a model for the role of intranuclear compartmentalization in eukaryotic DNA structure and function.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agard D. A., Sedat J. W. Three-dimensional architecture of a polytene nucleus. Nature. 1983 Apr 21;302(5910):676–681. doi: 10.1038/302676a0. [DOI] [PubMed] [Google Scholar]
  2. Bodnar J. W. A domain model for eukaryotic DNA organization: a molecular basis for cell differentiation and chromosome evolution. J Theor Biol. 1988 Jun 22;132(4):479–507. doi: 10.1016/s0022-5193(88)80086-9. [DOI] [PubMed] [Google Scholar]
  3. Bodnar J. W., Hanson P. I., Polvino-Bodnar M., Zempsky W., Ward D. C. The terminal regions of adenovirus and minute virus of mice DNAs are preferentially associated with the nuclear matrix in infected cells. J Virol. 1989 Oct;63(10):4344–4353. doi: 10.1128/jvi.63.10.4344-4353.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bodnar J. W., Jones G. S., Ellis C. H., Jr The domain model for eukaryotic DNA organization. 2: A molecular basis for constraints on development and evolution. J Theor Biol. 1989 Apr 6;137(3):281–320. doi: 10.1016/s0022-5193(89)80074-8. [DOI] [PubMed] [Google Scholar]
  5. Bodnar J. W., Pearson G. D. Kinetics of adenovirus DNA replication II. Initiation of adenovirus DNA replication. Virology. 1980 Sep;105(2):357–370. doi: 10.1016/0042-6822(80)90037-9. [DOI] [PubMed] [Google Scholar]
  6. Bourgeois C. A., Hubert J. Spatial relationship between the nucleolus and the nuclear envelope: structural aspects and functional significance. Int Rev Cytol. 1988;111:1–52. doi: 10.1016/s0074-7696(08)61730-1. [DOI] [PubMed] [Google Scholar]
  7. Hashimoto C., Steitz J. A. Sequential association of nucleolar 7-2 RNA with two different autoantigens. J Biol Chem. 1983 Feb 10;258(3):1379–1382. [PubMed] [Google Scholar]
  8. Hochstrasser M., Sedat J. W. Three-dimensional organization of Drosophila melanogaster interphase nuclei. II. Chromosome spatial organization and gene regulation. J Cell Biol. 1987 Jun;104(6):1471–1483. doi: 10.1083/jcb.104.6.1471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hutchison N., Weintraub H. Localization of DNAase I-sensitive sequences to specific regions of interphase nuclei. Cell. 1985 Dec;43(2 Pt 1):471–482. doi: 10.1016/0092-8674(85)90177-1. [DOI] [PubMed] [Google Scholar]
  10. Lawrence J. B., Villnave C. A., Singer R. H. Sensitive, high-resolution chromatin and chromosome mapping in situ: presence and orientation of two closely integrated copies of EBV in a lymphoma line. Cell. 1988 Jan 15;52(1):51–61. doi: 10.1016/0092-8674(88)90530-2. [DOI] [PubMed] [Google Scholar]
  11. Merchlinsky M. J., Tattersall P. J., Leary J. J., Cotmore S. F., Gardiner E. M., Ward D. C. Construction of an infectious molecular clone of the autonomous parvovirus minute virus of mice. J Virol. 1983 Jul;47(1):227–232. doi: 10.1128/jvi.47.1.227-232.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nakayasu H., Berezney R. Mapping replicational sites in the eucaryotic cell nucleus. J Cell Biol. 1989 Jan;108(1):1–11. doi: 10.1083/jcb.108.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pearson G. D., Hanawalt P. C. Isolation of DNA replication complexes from uninfected and adenovirus-infected HeLa cells. J Mol Biol. 1971 Nov 28;62(1):65–80. doi: 10.1016/0022-2836(71)90131-8. [DOI] [PubMed] [Google Scholar]
  14. Shahrabadi M. S., Roy K. L., Yamamoto T. Macromolecular content of inclusions produced by a canine adenovirus. J Virol. 1972 Oct;10(4):801–809. doi: 10.1128/jvi.10.4.801-809.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shiroki K., Shimojo H., Yamaguchi K. The viral DNA replication complex of adenovirus 12. Virology. 1974 Jul;60(1):192–199. doi: 10.1016/0042-6822(74)90376-6. [DOI] [PubMed] [Google Scholar]
  16. Shuman H., Murray J. M., DiLullo C. Confocal microscopy: an overview. Biotechniques. 1989 Feb;7(2):154–163. [PubMed] [Google Scholar]
  17. Simmons T., Heywood P., Hodge L. D. Intranuclear site of replication of adenovirus DNA. J Mol Biol. 1974 Nov 5;89(3):423–433. doi: 10.1016/0022-2836(74)90473-2. [DOI] [PubMed] [Google Scholar]
  18. Vlak J. M., Rozijn R. H., Spies F. Localization of adenovirus DNA replication in KB cells. Virology. 1975 Jun;65(2):535–545. doi: 10.1016/0042-6822(75)90058-6. [DOI] [PubMed] [Google Scholar]
  19. Voelkerding K., Klessig D. F. Identification of two nuclear subclasses of the adenovirus type 5-encoded DNA-binding protein. J Virol. 1986 Nov;60(2):353–362. doi: 10.1128/jvi.60.2.353-362.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Walton T. H., Moen P. T., Jr, Fox E., Bodnar J. W. Interactions of minute virus of mice and adenovirus with host nucleoli. J Virol. 1989 Sep;63(9):3651–3660. doi: 10.1128/jvi.63.9.3651-3660.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Zhai Z. H., Nickerson J. A., Krochmalnic G., Penman S. Alterations in nuclear matrix structure after adenovirus infection. J Virol. 1987 Apr;61(4):1007–1018. doi: 10.1128/jvi.61.4.1007-1018.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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