Table 2.
Mutations identified in R6M1 and R6M2 penicillin-resistant mutants
| S. pneumoniae strain | |||||
|---|---|---|---|---|---|
| M1 | M2 | ||||
| Locus name | Putative identification | Nucleotide change | Amino acid change | Nucleotide change | Amino acid change |
| spr0032 | DNA polymerase I | A107G | H36R | ||
| spr0041 | Transposase (orf2) | GAA31AAC | E11N | ||
| GCTCG36TCTCA | K12N;D14N | ||||
| T63A | SYN | ||||
| GG204TA | D69Y | ||||
| spr0113 | Hypothetical protein | A4G | K2E | ||
| spr0121 | Surface protein PspA precursor | A4G | N2D | ||
| T1374C | SYN | ||||
| spr0160 | DNA mismatch repair protein (Hex B) | G752A | S251N | ||
| spr0182 | Hypothetical protein | G82insertion | frame shift | ||
| spr0284 | Alpha-xylosidase | T1107C | SYN | ||
| spr0304 | Penicillin-binding protein 2x | A1150G | R384G | A842C | Q281P |
| G1552T | V518L | C1106T | A369V | ||
| C1654G | Q552E | A1150G | R384G | ||
| C1276T | R426C | ||||
| G1552A | V518I | ||||
| spr0329 | Penicillin-binding protein 1a | G1630A | G544R | G1233A | W411* |
| spr0376 | Conserved hypothetical protein | T207C | SYN | ||
| spr0422 | Hypothetical protein | C391T | Q131* | ||
| spr0475 | Conserved hypothetical protein | G318C | L106F | ||
| spr0509 | Phenylalanyl-tRNA synthetase beta chain | C105T | SYN | ||
| spr0598 | GTP-binding protein (TypA/BipA) (tyrosine phosphorylated protein A) | C905T | P302L | ||
| spr0666 | ABC transporter ATP-binding protein - cell division (FtsE) | C410T | P137L | ||
| spr0764 | 30S Ribosomal protein S1 | G1173A | M391I | ||
| spr0776 | D-Alanyl-D-alanine carboxypeptidase | C287T | A96V | ||
| spr0866 | Dihydroorotate dehydrogenase | C575T | P192L | ||
| spr0878 | Exoribonuclease R | G280T | G94W | ||
| spr0895 | Conserved hypothetical protein | G134C | R45T | ||
| spr0917 | Citrulline cluster-linked gene | C59T | A20V | ||
| spr0934 | ABC transporter substrate-binding protein - iron transport | G486A | W162* | ||
| spr1041 | Hypothetical protein | C648T | SYN | ||
| spr1043 | Conserved hypothetical protein | C109T | P37S | C105T | SYN |
| spr1092 | tRNA pseudouridine 5S synthase | C210T | SYN | ||
| spr1127 | Ribonuclease III | A228G | SYN | C554T | T185I |
| spr1152 | LicD | G692T | P231L | ||
| spr1166 | Signal recognition particle (Fifty four homolog) | C139T | P47S | ||
| spr1178 | Hypothetical protein | C82T | Q28* | C82T | Q28* |
| spr1186 | N-Acetylneuraminate lyase subunit, truncation | T603C | SYN | ||
| G620A | G207D | ||||
| GAC660AAT | T221I | ||||
| AA689CG | E230A | ||||
| T696A | SYN | ||||
| spr1224 | Conserved hypothetical protein | T564C | SYN | ||
| spr1240 | Alanyl-tRNA synthetase | A139G | T47A | ||
| spr1254 | ABC transporter ATP-binding protein-phosphate transport (PstB) | G499A | G167S | C614T | T205I |
| spr1260 | Conserved hypothetical protein | T597C | SYN | ||
| spr1272 | N-Acetylglucosamine-6-phosphate isomerase | G385A | G129R | ||
| spr1384 | UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine ligase | G996A | M332I | ||
| spr1423 | Conserved hypothetical protein | A673G | M225V | ||
| spr1453 | Major facilitator superfamily transporter | T91C | F31L | ||
| spr1465 | Conserved hypothetical protein | G419A | G140E | ||
| spr1517 | Penicillin-binding protein 2b | C1245A | D415E | C1184T | A395V |
| C1528A | G665D | G1303A | G435S | ||
| A1351G | T451A | ||||
| spr1587 | Conserved hypothetical protein | C568T | SYN | ||
| spr1703 | ABC transporter ATP-binding protein - oligopeptide transport | C575A | P192Q | ||
| spr1706 | ABC transporter membrane-spanning permease - oligopeptide transport | T399deletion | frameshift | ||
| spr1862 | Competence protein | A51G | SYN | ||
| spr1886 | Degenerate transposase | A314G | *105W | ||
| spr1888 | DNA mismatch repair protein (HexA) | C2183T | T728I | C976T | Q326* |
| spr1991 | Glycerol kinase | GG78TT | E27* | ||
Asterisks and SYN indicate nonsense mutations and synonymous mutations, respectively.