Figure 6.

Leukotrienes (LTs) have no impact on HIV-1 reverse transcripts, whereas they affect viral integration in monocyte-derived microglial-like cells (MDMis). Cells were first exposed to YU2 for 2 h. MDMis were then either left untreated (Ctrl) or treated with 10 ng/ml of LTB₄ or LTC₄. DNA was then extracted 8 h post infection for monitoring reverse transcribed products or 24 h post infection for assessing integration events. Quantification was achieved by real-time polymerase chain reaction (PCR). Amplified products were normalized using the housekeeping β-globin gene. To compensate for donor-to-donor variations, the results shown represent the means ± SD calculated from three independent experiments and are expressed as percentages of control (Ctrl) (*P < 0.05).