Table 3.
ΔmRNA for the housekeeping genes (ACTB, B2M, GAPDH, HPRT1, and RPL13A) in human bronchi after suspension in an organ bath with (5 minutes, 2
| No stretch | Stretch | |||
|---|---|---|---|---|
| Gene | Paired rings | ΔmRNA vs. Control H0 |
Paired rings | ΔmRNA vs. Control H0 |
| ACTB | 9 | 0.11 ± 0.58 | 9 | 0.01 ± 0.62 |
| B2M | 9 | -0.40 ± 0.46 | 9 | -0.40 ± 0.45 |
| GAPDH | 9 | 1.18 ± 0.42 | 9 | -0.88 ± 0.39 |
| HPRT1 | 9 | 0.14 ± 0.59 | 9 | -0.31 ± 0.61 |
| RPL13A | 9 | -0.07 ± 0.52 | 9 | 0.47 ± 0.50 |
After normalization to the mean of the five housekeeping genes, ΔmRNA for each target gene was calculated as the fold mRNA-value change of each non-stretched or stretched ring relative to the mRNA value in its paired control H0. Values are mean ± standard error of the mean. No significant differences in expression of house-keeping genes were observed. ACTB, β-actin; B2M, β2-microglobulin; GAPDH, glyceraldhyde-3-phosphate dehydrogenase; HPRT1, hypoxantine phosphoribosyltransferase 1; RPL13A, ribosomal protein L13α.