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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Nat Struct Mol Biol. 2012 Feb 19;19(3):361–363. doi: 10.1038/nsmb.2252

Figure 3.

Figure 3

Set5 functions with COMPASS and NuA4 complexes to regulate cell growth and stress responses. (a) Decreased fitness of set5Δ set1Δ upon stress. Four-fold serial dilutions of yeast were spotted and grown for 2–5 days at 30°C. (MMS: methylmethane sulfonate; HU: hydroxyurea) (b) Mutation of the conserved Y402 to alanine abolishes Set5’s catalytic activity. Methylation assay with wild type or Y402A Set5 on rH4 and native histones. (c) The catalytic activity of Set5 is required for normal growth of set1Δ cells under stress. Deletion (d) or catalytic inactivation (e) of SET5 in yng2Δ strains impairs cell growth.