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. 2012 Mar 8;73(5):951–961. doi: 10.1016/j.neuron.2011.12.039

Figure 2.

Figure 2

Localization of ClC-2 and GlialCAM in the Brain

Mouse brain sections labeled with antibodies against GlialCAM, ClC-2, or GFAP antibodies. (A) cerebellar Bergmann glia; (B) astrocytic endfeet surrounding blood vessels in cortex; (C and D) EM immunolabeling of human cerebellum shows localization of ClC-2 (arrows) in astrocyte-astrocyte junctions near basal lamina; (E) oligodendrocytic cell bodies in myelinated fibers of cerebellar white matter tracts.

(F) Coexpression of GlialCAM and the oligodendrocyte marker Cx47 in membranes of oligodendrocytic bodies in the cerebellum.

(G) EM immunolabeling detects ClC-2 in myelin (arrows).

Scale bars for (A), (B), and (E) 20 μm; (F) 5 μm; for panels (C) and (D) 200 nm; (G) 500 nm. M: myelin; Ax: axon; Ast: astrocyte; BL: basal lamina. ClC-2 antibodies used: C1 (A); C2 (B–F). See also Figure S2.