Figure 8.

ClC-2 and GlialCAM Subcellular Localization Changes Caused by GLIALCAM Mutations in Primary Cultures of Astrocytes

(A–E) Astrocytes were cotransduced with adenoviruses expressing ClC-2 together with wild-type GlialCAM (A) or containing the MLC-related mutations R92Q (B), R98C (C), R92W (D), and G89D (E). Cells were fixed, permeabilized, and then immunofluorescence was performed with a rabbit polyclonal antibody against ClC-2 (green) and a monoclonal antibody detecting GlialCAM protein (red). Nuclei were stained with DAPI (blue). Colocalization between the green and the red channel is shown in yellow. Images correspond to representative cells from three independent experiments. Scale bar: 20 μm.