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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Methods Mol Biol. 2012;832:505–518. doi: 10.1007/978-1-61779-474-2_36

Fig. 1.

Fig. 1

The cycloheximide chase analysis of CFTR-HA (a) and CPY*HA (b) was performed as described in Subheading 3.1 using a strain that contains a thermo-sensitive allele in the CDC48 gene (cdc48-10) and in an isogenic wild type. Western blots were probed with anti-HA antibody to detect substrate and anti-Sec61p antibody was used as a loading control