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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Methods Mol Biol. 2012;832:505–518. doi: 10.1007/978-1-61779-474-2_36

Fig. 2.

Fig. 2

The cycloheximide chase analysis of CFTR-HA was performed as described in Subheading 3.1 using a strain lacking the multidrug resistance pump, PDR5. Cells were incubated with MG132 for 1 h prior to the cycloheximide chase (see Note 6). Western blots were probed with anti-HA antibody to detect the CFTR-HA substrate and with anti-Sec61p antibody as a loading control.