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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Methods Mol Biol. 2012;832:505–518. doi: 10.1007/978-1-61779-474-2_36

Fig. 3.

Fig. 3

The in vivo ubiquitylation assay was performed as described in section 3.2. CFTR-HA was immunoprecipitated with anti-HA-conjugated agarose from a strain lacking both the Hrd1p and Doa10p E3 ubiquitin ligases and an isogenic wild-type strain. The immunoprecipitated CFTR-HA was resolved on a 10% denaturing polyacrylamide gel and probed with anti-ubiquitin and anti-HA antibodies.