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. 2012 Apr 24;2:379. doi: 10.1038/srep00379

Figure 2. Mapping of the domains responsible for the interaction between Mieap and 14-3-3γ.

Figure 2

(a) The Mieap and 14-3-3γ deletion mutants. The numbers of amino acid residues are indicated. (b and c) Mieap interacts with 14-3-3γ via the N-terminal region of Mieap. The HCT116 cells were co-transfected with FLAG-tagged 14-3-3γ and various HA-tagged Mieap mutants and were γ-irradiated. Seventy-two hours after the IR, the cell lysates were immunoprecipitated with anti-HA antibody. An immunoblot analysis was performed using the anti-FLAG or anti-HA antibody. α: Mieap α, β: Mieap β, IP: immunoprecipitation, lysate: total cell lysate, and IB: immunoblotting. (d) 14-3-3γ interacts with Mieap β via the PB domain of 14-3-3γ. The HCT116 cells were co-transfected with HA-tagged Mieap and various FLAG-tagged 14-3-3γ mutants and were γ-irradiated. Seventy-two hours after the IR, the cell lysates were immunoprecipitated with anti-HA antibody. An immunoblot analysis was performed using the anti-FLAG or anti-HA antibodies. IP: immunoprecipitation, lysate: total cell lysate, and IB: immunoblotting.