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. 2012 Apr 24;2:379. doi: 10.1038/srep00379

Figure 5. Potential role of 14-3-3γ in eliminating oxidized proteins within mitochondria.

Figure 5

(a and b) An IF analysis of MALM and oxidized proteins. The Ad-Mieap-β- or Ad-LacZ-infected control and Ad-Mieap-β-infected 14-3-3γ KD HCT116 cells (a) or the control, Mieap-KD, and 14-3-3γ KD A549 cells (b) were γ-irradiated. Three days after the IR, an IF analysis was performed using anti-LAMP1 antibody (LAMP1) to detect MALM (MALM) or using anti-nitrotyrosine antibody (Nitrotyrosine) to detect nitrotyrosine-oxidized proteins (Oxidized proteins). To evaluate the role of lysosomes, lysosome enzyme inhibitors were added to the A549 control and Ad-Mieap-β-infected HCT116 control cells on day 2 after the IR. The mitochondria are indicated by the DsRed-mito protein signal (Mito). Representative images are shown (upper panel of a or b). A quantitative analysis of MALM or nitrotyrosine intensity was performed using 300-400 cells. The average intensities of the MALM or nitrotyrosine-oxidized proteins per cell are shown with error bars indicating 1 standard deviation (SD; lower panel). P < 0.01 (*) was considered statistically significant. Scale bar = 20 μm.