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. 2012 Apr 19;8(4):e1002633. doi: 10.1371/journal.ppat.1002633

Figure 3. Reduction in immunogenicity as a result of point mutation in the LCMV NP205 epitope (NP V207A).

Figure 3

(A) B6 mice (3/group) were inoculated with either rLCMV WT or rV207A variant LCMV-Armstrong. Eight days PI, splenocytes from each group were harvested and stimulated ex vivo with a panel of LCMV-specific CD8 T cell peptides for ICS assays. Numbers represent frequencies of IFNγ+, CD8α+ T cells. (B) Splenocytes from rWT- or rV207A-infected mice 8 days PI were stained with LCMV NP205 WT and NP V207A peptide-loaded MHC-Ig dimers. (C) B6 mice (3/group) were inoculated with rLCMV WT or rL212A viruses. Spleens were harvested 8 days PI and stimulated ex vivo with indicated peptides. Numbers represent frequencies of IFNγ+, CD8α+ T cells in representative mice.