Figure 2.

Sites of circular permutation BLA based on DNA sequencing of (A) 25 randomly selected members from the naïve library, (B) 20 randomly selected members capable of growing on plates containing 16 µg/ml ampicillin, and (C) 10 randomly selected members capable of growing on plates containing 250 µg/ml ampicillin. The first amino acid in the mature wild-type BLA is 24 (since the signal sequence of amino acids 1–23 is removed) and the last amino acid is 286. The linker joining the N- and C-termini has the amino acid sequence DKS.