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. 2012 Apr 19;8(4):e1002663. doi: 10.1371/journal.ppat.1002663

Figure 2. Brg1 interacts with Hda1 in vivo and binds to promoters of hypha-specific genes.

Figure 2

(A). Cells of wild type transformed with Myc-Brg1 and Hda1-Protein A (HLY4081) or with only Myc-Brg1 (HLY3636) were grown in YEPMaltose medium at 25°C or 37°C in the presence or absence of 10 nM rapamycin. Protein lysates were subjected to immunoprecipitation with IgG beads (Sigma). The precipitated proteins (IP) and cell lysates (input) were analyzed by Western blotting. (B). Brg1 is present at the HWP1 promoter in a rapamycin-containing medium. Cells of wild-type strain carrying Brg1-Myc (HLY4082) were diluted into YPD medium at 37°C in the presence or absence of 10 nM rapamycin. ChIP DNA was quantitated by qPCR as described in Figure 1. The ChIP data show the average of three independent qPCR experiments with error bars representing the SEM.