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. 2012 Apr 19;8(4):e1002663. doi: 10.1371/journal.ppat.1002663

Figure 6. Ume6 functions downstream of Brg1 and Hda1 in hyphal maintenance.

Figure 6

(A). Ume6 is not essential for promoter recruitment of Hda1 during hyphal elongation. ChIP of Hda1-Myc in wild type, brg1, and ume6 mutants in YPD with 10 nM rapamycin at 37°C. (B). UME6 expression requires Brg1 and Hda1. Expression levels were quantified by qRT-PCR and normalized with ACT1. All data show the average of three independent qRT-PCR or qPCR experiments with error bars representing the SEM. (C). Ume6 binds to hyphal promoters during hyphal elongation. ChIP and Western analysis of Ume6 in wild type cells (HLY4078) in indicated media. (D). Ectopic expression of UME6, but not BRG1, restores filamentous growth in the hda1 mutant. hda1 cells transformed with pBES116 (V), MAL2p-MYC-BRG1 (HLY4077) or MAL2p-UME6-MYC (HLY4076) were grown in YEPMaltose medium with 5 nM rapamycin for cell morphology.